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pY588Y594 builds up as a result of dephosphorylation at Y594 on the doubly phosphorylated peptide ppY588Y594 as the reaction proceeds. 10 μM EphA2 C0 was treated with 10 μM HCPTP-A (C0 + A 10:10) or with 1 μM HCPTP-A (C0 + A 10:1) and the SRM-MS data for pY588Y594 was analyzed at various timepoints. The mean and standard deviation of three independent replicates are plotted.
