Table I.
Phosphopeptide Mapping of EphA2 C0 by LC-MSMS
| Tyrosine | Domains | Peptide sequencesa |
|---|---|---|
| 575 | Jm | 569-QSPEDV[Y]FSK−578 |
| 588b | 587-T[Y]VDPHTYEDPNQAVLK−603 | |
| 594b | 587-TYVDPHT[Y]EDPNQAVLK−603 | |
| 628 | Kinase | 618-VIGAGEFGEV[Y]KGMLK−633 |
| 694 | 685-YKPMMIITE[Y]MENGALDK−702 | |
| 735 | 729-YLANMN[Y]VHR−738 | |
| 772 | 763-VLEDDPEAT[Y]TTSGGK−778 | |
| 921 | SAM | 918-MQQ[Y]TEHFMAAGYTAIEK−935 |
| 960 | 958-IA[Y]SLLGLK−966 |
a
Peptide sequences represent the tryptic peptides with phosphorylated tyrosines indicated within brackets detected by mass spectrometry.
b
Tyrosines 588 and 594 are present on the same tryptic peptide and the doubly phosphorylated and both singly phosphorylated forms were detected.