Table II.
Measurement of Phosphorylation Stoichiometry for Each Tyrosine Monitored Using SRM-MS
| EphA2 Peptidesa | Peptide nomenclatureb | Measured Stoichiometry (%) | pY Content [S0] (μM) |
|---|---|---|---|
| QSPEDV[Y575]FSK | pY575 | 70 ± 5 | 7.0 |
| T[Y588]VDPHT[Y594]EDPNQAVLK | ppY588Y594 | 47 ± 10 | 4.7 |
| T[Y588]VDPHTY594EDPNQAVLK | pY588Y584 | 17 ± 4 | 1.7 |
| TY588VDPHT[Y594]EDPNQAVLK | Y588pY594 | 29 ± 7 | 2.9 |
| VLEDDPEAT[Y772]TTSGGK | pY772 | 80 ± 2 | 8.0 |
| IA[Y960]SLLGLK | pY960 | 8 ± 2 | 0.8 |
a
Phosphorylated tyrosines are indicated within brackets in the peptide sequence.
b
The peptides are referred to by this naming system in all figures and tables.