Figure 1.

Identification of epitope M133-specific T reg cells using MHC class II tetramer. (A) CNS-derived lymphocytes from rJ2.2-infected Foxp3^gfp mice were analyzed for Foxp3⁺ CD4 T cells at the indicated times after infection (n = 5 mice for each time point, in at least two independent experiments). (B) BrdU incorporation by CNS-derived CD4 T cells at 6 d after infection is shown. Data shown are representative of six mice analyzed in three independent experiments. (C and D) Foxp3^gfp mice were infected with rJ2.2 (C) or rJ2.2.M_Y135Q (D) and sacrificed at the indicated times after infection. Brain and spinal cord (SC)–derived lymphocytes were stained with I-A^b/M133 tetramers and representative plots gated on CD4⁺ T cells are shown. (E) Frequency of T reg cells that are I-A^b/M133 tetramer-positive is shown. Data are from four to seven mice per time point in two to five independent experiments. Error bars in A and E indicate mean ± SEM.