Table I.
Patient no. | Loss of E2A | Purity of enriched tumor cells | |
Array CGH analysis | FISH analysis (number of cells with deletion/number of analyzed cells) | ||
% | |||
1 | yesc | yes (149/200)e | 96.7 |
2 | yesc | n.d | – |
3 | yesc | yes (198/200)e | 99.1 |
4a | yesc | yes (167/200)e | 93.2 |
5 | yese | yes (90/200)e | 97.9 |
6 | yesd | n.d. | – |
7a | yesc | no (14/200)e | 99.2f |
8 | yese | n.d. | – |
9 | noc | no (3/200)e | 97.4 |
10 | noc | no (4/200)e | 96.9 |
11a | yese | no (12/200)d | n.d. |
12 | noc | n.d. | – |
13 | yesc | yes (126/200)e | 98.4f |
14 | noc | n.d. | – |
15a | nobc | yes (106/200)d | n.d. |
16a | noe | yes (loss 28/200, gain 46/200)e | 97.2 |
17 | yese | yes (50/200)e | 98.4 |
18 | yesc | n.d. | – |
19 | yese | n.d. | – |
20 | yese | n.d. | – |
Se-Ax cell line | yes | yes (190/200) |
n.d.: not determined
CGH and FISH analyses were performed with samples from different time points (see Table S1).
hypotriploid cells, gain of chromosome 19 except 19p13.2-13.3.
Analysis of PBMC cells.
Analysis of CD4-sorted cells.
Analysis of Vβ-sorted cells.
Purity was determined with CD4 antibody only.