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. 2011 Aug 1;208(8):1595–1603. doi: 10.1084/jem.20101691

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© 2011 Gutierrez et al.

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Figure 6. — Expression of activated Akt does not alter the transcriptional activity of MYC. (A) Whole mount RNA in situ hybridization for ddx18, a known direct target of MYC in mammalian cells (Grandori et al., 1996; O’Hagan et al., 2000), performed at the 24-h postfertilization developmental stage in zebrafish embryos that were injected with 100 pg mCherry (control) or Myc mRNA at the one-cell stage. One representative zebrafish is shown in each condition out of a minimum of 20 embryos analyzed per condition. (B) Q-RT-PCR for ddx18 expression, performed using RNA from T-ALL cells from rag2:MYC-ER;rag2:EGFP-bcl2 zebrafish which also expressed either a rag2:GFP or a rag2:myr-mAkt2 transgene. T-ALL cells were sorted from animals in 4HT (+4HT) or 4 d after 4HT removal (−4HT). β-Actin was used as the Q-RT-PCR control. Bcl2-transgenic T-ALL cells were used in all conditions to avoid comparing live versus dying cells after MYC-ER inactivation. Error bars represent standard error of the mean. Number of tumors analyzed per group: 4HT+, myr-mAkt2−, n = 6; 4HT−, myr-mAkt2−, n = 7; 4HT+, myr-mAkt2+, n = 5; 4HT−, myr-mAkt2+, n = 5.