Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 1;208(8):1721–1735. doi: 10.1084/jem.20101920

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Schnoor et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Reduced leukocyte transmigration after down-regulation of cortactin is accompanied by impaired formation of ICAM-1 clusters. HUVECs were incubated for 20 min with human primary PMNs as described in Materials and methods followed by fixation and immunofluorescence microscopy. (A) HUVECs transfected with control (ctrl) siRNA show ICAM-1 (red) and cortactin (green) containing ring structures surrounding a PMN. HUVECs transfected with cortactin-specific siRNA show no ICAM-1 clustering around the PMN. Associated leukocytes were visualized by transmitted light (left). Bar, 5 µm. (B) Apically adherent PMNs from randomly selected fields of three independent experiments were scored for the presence of ICAM-1–containing ring structures. A representative experiment with the percentage of analyzed cells is displayed. A total of 112 PMNs (on cortactin-depleted HUVECs) and 119 PMNs (on cortactin-positive HUVECs) were analyzed.