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. 2011 May 4;100(9):2141–2150. doi: 10.1016/j.bpj.2011.03.015

Figure 4.

Figure 4

Docking and fusion versus time in tethered-vesicle assay. Percentage of tethered v-SNARE vesicles that docked but did not fuse with a t-SNARE vesicle (gray data points) or docked and fused with a t-SNARE vesicle (black data points) at each reaction time, as determined by the absolute FRET efficiency. The t-SNARE vesicle concentration was 10 nM. (a) Without VC peptide. (c) In the presence of 5 μM VC peptide. Docked but unfused curves are corrected for false colocalizations between a tethered v-SNARE vesicle and a t-SNARE vesicle (see SupportingMaterial). The total docking curves in panels b and d are the sum of the docked but unfused plus the docked and fused curves in panels a and c. Docked but unfused curves are corrected for false colocalizations between a v-SNARE vesicle and a t-SNARE vesicle (see Supporting Material). The dotted lines represent the linear fits used to determine kdock,teth.