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Histogram of τfus for single-vesicle fusion events with VC peptide. The histogram shows the dwell time in the docked but unfused state for 36 fusion events observed in real time with the tethered vesicle assay in the presence of VC peptide at 5 μM. Data were obtained with 3 s time resolution. See Fig. S9 for six examples of real-time FRET traces. Movie S1 shows an example of a single fusion event.
