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. 1966 Feb;91(2):750–754. doi: 10.1128/jb.91.2.750-754.1966

Cationic Proteins of Polymorphonuclear Leukocyte Lysosomes I. Resolution of Antibacterial and Enzymatic Activities

H I Zeya a, J K Spitznagel a,1
PMCID: PMC314924  PMID: 5934973

Abstract

Zeya, H. I. (University of North Carolina, Chapel Hill), and J. K. Spitznagel. Cationic proteins of polymorphonuclear leukocyte lysosomes. I. Resolution of antibacterial and enzymatic activities. J. Bacteriol. 91:750–754. 1966.—A lysosomal fraction from polymorphonuclear (PMN) leukocytes of guinea pig peritoneal exudate was subjected directly to electrophoresis on cellulose acetate paper treated with cetyltrimethyl ammonium bromide. The Iysosomal components resolved into seven bands moving towards the cathode. Assay of the eluted bands showed that the antibacterial activity was distinct from lysosomal enzymes and was associated with three cationic components (bands I, II, and III) which migrated most rapidly towards the cathode, ahead of lysozyme ribonuclease and deoxyribonuclease. Qualitatively, the antibacterial components appeared to be rich in arginine. The antibacterial components were absent in the pherograms of nuclear fractions of PMN leukocytes and in supernatant fractions that remained after lysosomes were removed from cell homogenates by centrifugation at 8,000 × g.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATHENS J. W., MAUER A. M., ASHENBRUCKER H., CARTWRIGHT G. E., WINTROBE M. M. Leukokinetic studies. I. A method for labeling leukocytes with diisopropyl-fluorophosphate (DFP32). Blood. 1959 Apr;14(4):303–333. [PubMed] [Google Scholar]
  2. COHN Z. A., HIRSCH J. G. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:983–1004. doi: 10.1084/jem.112.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HIRSCH J. G. Bactericidal action of histone. J Exp Med. 1958 Dec 1;108(6):925–944. doi: 10.1084/jem.108.6.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kanthack A. A., Hardy W. B. The Morphology and Distribution of the Wandering Cells of Mammalia. J Physiol. 1894 Aug 13;17(1-2):80.1–8119. doi: 10.1113/jphysiol.1894.sp000520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. SCHNEIDER W. C., HOGEBOOM G. H. Intracellular distribution of enzymes. X. Desoxyribonuclease and ribonuclease. J Biol Chem. 1952 Sep;198(1):155–163. [PubMed] [Google Scholar]
  6. SHUGAR D. The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme. Biochim Biophys Acta. 1952 Mar;8(3):302–309. doi: 10.1016/0006-3002(52)90045-0. [DOI] [PubMed] [Google Scholar]
  7. SKARNES R. C., WATSON D. W. Antimicrobial factors of normal tissues and fluids. Bacteriol Rev. 1957 Dec;21(4):273–294. doi: 10.1128/br.21.4.273-294.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SPITZNAGEL J. K., CHI H. Y. CATIONIC PROTEINS AND ANTIBACTERIAL PROPERTIES OF INFECTED TISSUES AND LEUKOCYTES. Am J Pathol. 1963 Oct;43:697–711. [PMC free article] [PubMed] [Google Scholar]
  9. TISELIUS A., FLODIN P. Zone electrophoresis. Adv Protein Chem. 1953;8:461–486. doi: 10.1016/s0065-3233(08)60097-2. [DOI] [PubMed] [Google Scholar]
  10. TISELIUS A., FLODIN P. Zone electrophoresis. Adv Protein Chem. 1953;8:461–486. doi: 10.1016/s0065-3233(08)60097-2. [DOI] [PubMed] [Google Scholar]
  11. ZEYA H. I., SPITZNAGEL J. K. ANTIBACTERIAL AND ENZYMIC BASIC PROTEINS FROM LEUKOCYTE LYSOSOMES: SEPARATION AND IDENTIFICATION. Science. 1963 Nov 22;142(3595):1085–1087. doi: 10.1126/science.142.3595.1085. [DOI] [PubMed] [Google Scholar]

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