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. 2011 May 4;100(9):2201–2208. doi: 10.1016/j.bpj.2011.03.023

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Histograms of tRNA rupture force from the ribosome. All the measurements were done at 4 mM [Mg²⁺] except for those in panel D. (A) Phe-tRNA^Phe rupture from the A-site of the 30S subunit containing mRNA and tRNA^fMet in the P-site. (B) Phe-tRNA^Phe rupture from the A-site of the ribosome containing mRNA and tRNA^fMet in the P-site. (C) AcPhe-tRNA^Phe rupture from the A-site of the ribosome containing mRNA and tRNA^fMet in the P-site. (D) Phe-tRNA^Phe rupture from the A-site of the ribosome containing mRNA and tRNA^fMet in the P-site at 15 mM [Mg²⁺]. (E) Phe-tRNA^Phe rupture from the A-site of the ribosome containing mRNA and tRNA^fMet in the P-site in presence of viomycin. (F) Phe-tRNA^Phe rupture from the ribosome containing mRNA and fMet-tRNA^fMet after incubating with EF-G/GTP.