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. 2011 May 4;100(9):2077–2083. doi: 10.1016/j.bpj.2011.03.020

Figure 1.

Figure 1

FACE gels for determination of (A) HS and (B) HA and CS content. Lane 1: standard; lane 2: BLC; lane 3: AMAC reaction buffer. Band positions for ΔU-G-NAc ([ΔUA-glcNAc]), ΔU-G-NS ([ΔUA-glcNAc(2-N-sulfate)]), ΔU-G(6S)-NAc ([ΔUA-glcNAc(6-O-sulfate)]), ΔU(2S)-G-NS ([ΔUA(2-O-sulfate)-glcNAc(6-O-sulfate)]), ΔU-G(6S)-NS ([ΔUA-glcN(2-N-, 6-O-sulfate)]), and ΔU(2S)-G(6S)-NS ([ΔUA(2-O-sulfate)-glcN(2-N-, 6-O-sulfate)]) are indicated beside the gel in panel A. Band positions for ΔDiHA ([ΔUA-glcNAc]), ΔDi0S ([ΔUA-galNAc]), ΔDi6S ([ΔUA-galNAc(6-O-sulfate)]) and ΔDi4S ([ΔUA-galNAc(4-O-sulfate)]), ΔDi2S ([ΔUA(2-O-sulfate)-galNAc]), ΔDi4,6S ([ΔUA-galNAc(4-O-, 6-O-disulfate)]), and ΔDi2,6S ([ΔUA(2-O-sulfate)-galNAc(6-O-sulfate]) are indicated beside the gel in panel B.