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. 2011 Apr 1;145(1):79–91. doi: 10.1016/j.cell.2011.02.047

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© 2011 ELL & Excerpta Medica.

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Figure S3 — iRhoms Reduce Secretion and Intracellular Levels of Multiple EFGR Ligands, Related to Figure 6

(A–E) Secretion (sup, upper panel) and intracellular levels (extr, second panel from top) of the indicated EGFR ligands were decreased by human iRhom1 (iR1-HA) and mouse iRhom2 (iR2-HA) but unaffected by mouse Unc93B. Secretion and intracellular levels of Flag-tagged prolactin was unaffected by iR1, iR2 and Unc93B (E). EGFR ligands, and prolactin were detected with myc and Flag antibodies, respectively. Levels of iRhom1, iRhom2 and Unc93B were monitored by anti-HA antibodies in the cell extracts (extr, third panel from top). In all panels blots were probed with anti-actin to control for equal loading (extr, lower panel).

(F) In HeLa cells intracellular levels of myc-tagged EGF was decreased by human iRhom1 (iR1-HA) whereas levels of Flag-tagged Delta were unaffected by iR1-HA. EGF, and Delta were detected with myc and Flag antibodies, respectively. Levels of iR1 were monitored by anti-HA antibodies in the cell extracts. In all panels, blots were probed with anti-actin to control for equal loading.