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. 2011 Jul 23;7(6):892–901. doi: 10.7150/ijbs.7.892

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 2 — Identification and characterization of T1 cell line. Cell cycle and PCNA analysis indicated that the percentages were 42.60% (A) for the S phase cells and 91.46% positive for PCNA (B) respectively in T1 cells. Immunofluorescence assay showed the image of nuclear staining, using an anti-human nucleus antibody (C). Nuclei were stained with DAPI (Scale bar: 100 μm). DNA fingerprinting with the STR markers of the donor hsNSCs (passage 22, 28 respectively) and the T1 cells was examined and compared (D-F). The boxed numbers and corresponding peaks of the hsNSCs at passage 22 and 28 were completely identical, but were significantly different from those of the T1 cells.