FIGURE 1.

Glucose regulates Hmg1 phosphorylation. A, wild-type cells growing exponentially in YES medium were collected by centrifugation and resuspended in glucose-free medium. Samples were taken at the indicated times, and Hmg1 immunoprecipitates (IP) were blotted using anti-Hmg1 IgG or Hmg1 phosphospecific antibodies. B, wild-type cells were grown to exponential phase overnight in rich YES medium, collected by centrifugation, and shifted to glucose-free medium for 30 min. At time 0, cells were again collected by centrifugation and transferred to glucose-rich medium. Samples were taken at the indicated times and processed as in A. C, wild-type cells growing exponentially in YES medium were collected by centrifugation and resuspended in glucose-free medium for 30 min. The indicated carbon sources (30 g/liter) were added for 15 min. Cells were harvested by centrifugation and processed as in A.