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. 2011 Jun 7;286(31):27197–27205. doi: 10.1074/jbc.M111.237412

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — A, correlation between the values of ΔG_act and R₅₀ for double mutations containing L596G. Values of R₅₀ for L596G double mutants were obtained as described under “Experimental Procedures” and are reported as a function of their associated ΔG_act. The linear regression was calculated with the routine included in Origin 7.0 and showed a strong negative correlation between these two values, with a R factor of −0.95. B, correlation between the values of ΔG_act and R₅₀ for double mutations containing I701G. Values of R₅₀ for I701G double mutants were obtained as described under “Experimental Procedures” and are reported as a function of their associated ΔG_act. The L596G/I701G mutant is indicated by a asterisk. The double mutant S598G/I701G is shown but not identified on the graph. The linear regression was calculated with the routine included in Origin 7.0 and showed a strong negative correlation between these two values, with a R factor of −0.85.