TABLE 1.

PCR primers and conditions

Gene	Strand	Primer sequences	Size (bp)	ATa	Origin	Detection
			bp	°C
Cytl1	Sb	5′-AGATCACCCGCGACTTCAAC-3′	302	60	Human	RT-PCR
	Asc	5′-TTAGCGCTGACGATCTGGC-3′
	S	5′-CCACCTGCTACTCTCGGATG-3′	309	50	Mouse	RT-PCR
	As	5′-CCTCGGGAATTGGGTCTTC-3′
	S	5′-GATTCCTGTGTGAGGTAC-3′	342	60	Mouse	Genotyping (WT)
	As	5′-CAGAAGGAGTTCATGATG-3′
	S	5′-GAGGATCTCGTCGTGAC-3′	615	60	Mouse	Genotyping (KO)
	As	5′-CAGAAGGAGTTCATGATG-3′
Col2a1	S	5′-GGGTCTCCTGCCTCCTCCTGCTC-3′	204	62	Mouse	RT-PCR
	As	5′-TCCTTTCTGCCCCTTTGGCCCTAATTTTCGGG-3′
Acan	S	5′-GAAGACGACATCACCATCCAG-3′	581	56	Mouse	RT-PCR
	As	5′-CTGTCTTTGTCACCCACACATG-3′
Lect1	S	5′-ACAGTGACCAAGCAGAGCATC-3′	504	62	Mouse	RT-PCR
	As	5′-GCGGCATCCTTGGTAATTG-3′
Sox9	S	5′-GCGCGTGCAGCACAAGAAGGACCACCCGGATTACAAGTAC-3′	385	50	Mouse	RT-PCR
	As	5′-CCTCGGGAATTGGGTCTTC-3′
Gapdh	S	5′-CGTCTTCACCACCATGGAGA-3v	300	62	Human	RT-PCR
	As	5′-CGGCCATCACGCCACAGTTT-3′
	S	5′-TCACTGCCACCCAGAAGAC-3′	450	60	Mouse	RT-PCR
	As	5′-TGTAGGCCATGAGGTCCAC-3′

^a A_T, annealing temperature.

^b S, sense primer.

^c As, antisense primer.