TABLE 2.

Kinetic parameters of seeded Tau fibrillation

Kinetic traces of Tau fibrillation are shown in Fig. 4 and fitted to the sigmoidal function (see Equation 1). Errors are estimated by three independent experiments.

Soluble Tau	Seeds	Ffinal	t½	k
			min	min−1
1N3RCA	1N3RCA	(3.21 ± 0.10) × 103	(1.26 ± 0.05) × 101	(9.41 ± 0.51) × 10−2
	1N3RS-S	(2.68 ± 0.06) × 103	(2.23 ± 0.06) × 102	(6.09 ± 0.10) × 10−3
	1N4RCA	N.D.a	N.D.a	N.D.a
	1N4RS-S	N.D.a	N.D.a	N.D.a
1N4RCA	1N3RCA	(1.90 ± 0.06) × 103	(3.09 ± 0.03) × 102	(3.27 ± 0.02) × 10−3
	1N3RS-S	(5.00 ± 0.09) × 103	(7.07 ± 0.06) × 102	(5.50 ± 0.09) × 10−3
	1N4RCA	(1.51 ± 0.03) × 103	(1.83 ± 0.09) × 100	(2.35 ± 0.03) × 10−1
	1N4RS-S	(2.54 ± 0.11) × 103	(3.77 ± 0.12) × 102	(6.82 ± 0.17) × 10−3
1N4RS-S	1N3RCA	(1.01 ± 0.05) × 103	(2.62 ± 0.29) × 102	(5.06 ± 0.41) × 10−3
	1N3RS-S	(7.53 ± 0.22) × 102	(1.76 ± 0.26) × 102	(3.54 ± 0.30) × 10−3
	1N4RCA	(5.17 ± 0.22) × 102	(3.26 ± 0.13) × 102	(4.05 ± 0.14) × 10−3
	1N4RS-S	(2.17 ± 0.03) × 103	(1.17 ± 0.01) × 101	(1.10 ± 0.01) × 10−1

^a Not determined. A seeding reaction occurs very slowly within the observed time scale (also see Fig. 4A).