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Design, expression, and estimation of oligomer formation of m01s. A, amino acid sequence alignment of wide-type CH2 (NCBI accession number J00228), m01, and m01s. B, comparison of the expression of CH2, m01, and m01s. M, marker. C, size exclusion chromatography of m01s. The inset is a standard curve. mAU, milli-absorbance units.
