FIGURE 3.

[³H]TCA uptake ratio under equilibrating (12 mm) versus saturating (137 mm) sodium concentrations. Uptake of 5 μm TCA (spiked with [³H]TCA) in COS-1 cells transfected with C270A or mutant plasmid, in pmol [³H]TCA internalized per minute per milligram of protein, calculated as the ratio of uptake at 12 mm/137 mm sodium concentrations, and further normalized as a percentage of the parental C270A ratio. Bars represent the S.E. of three independent experiments, each performed in triplicates. ANOVA with p < 0.05 (*) indicate significant mean differences.