FIGURE 4.

MTSET labeling of TM1 mutants. Transiently transfected COS-1 cells were preincubated with 1 mm MTSET for 10 min, at room temperature, followed by washes, and [³H]TCA uptake. The ratio of uptake in presence versus absence of MTSET (i.e. MTSET/Initial rates of uptake) was expressed as a percentage of MTSET/Initial ratio of the parental C270A. Bars represent the S.E. of three independent experiments, each performed in triplicates. ANOVA with p < 0.05 (*) indicate significant mean differences.