FIGURE 5.

SUMO modification of casein kinase II α. A, substrates of casein kinase II. B, SUMOplot and SUMOsp 2.0 prediction of the casein kinase II α subunit. The stringency of SUMOsp 2.0 was set to be at high stringency. C, SUMO modification of casein kinase II α. The HA-tagged casein kinase II α subunit was expressed in 293T cells. After 24-h transfection, cells were lysed and immunoprecipitated (IP) with anti-HA antibody and then probed with anti-SUMO2/3 antibody. Total lysates of transfected cells were blotted with anti-HA antibody and anti-actin antibody. IB, immunoblotting.