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. 2011 Jun 15;286(31):27406–27415. doi: 10.1074/jbc.M111.229047

FIGURE 5.

FIGURE 5.

A, single-molecule FRET histograms of the G39-C65 aptamer as a function of Mg2+. The two-Gaussian fits are shown for each histogram. Individual time traces were manually filtered to remove blinking and photobleaching events. B, representative single-molecule FRET trajectories (33 ms of time resolution) at the specified Mg2+ concentrations. Corresponding FRET histograms for each trajectory are shown on the right. C, scatter plot showing the average duration (Av. duration) of U and F states from 300 molecules at 50 μm Mg2+. D and E, dwell time histograms of the unfolded (D) and folded state (E) in the presence of 50 μm Mg2+. The data have been fitted to single-exponential decay functions to give the rates of folding (kfold) and unfolding (kunfold).