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Cu2+ inhibits native human ENaCs in human airway epithelial cells. A, superimposed short circuit recordings showing the responses of the currents to 1, 10, 30, and 100 μm Cu2+ applied in apical chamber. B, ICu/I, ratio of amiloride-sensitive current in the presence of Cu2+ and amiloride-sensitive current prior to Cu2+ application. The asterisks indicate that the values in the presence of 30 and 100 μm Cu2+ were significantly less than that of base-line currents (p < 0.001, n = 6).
