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. 2011 Jun 9;286(31):27506–27514. doi: 10.1074/jbc.M111.238501

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — PKR engagement is required for activation of ERK1/2 or JNK in response to TNFα or IFNγ. A, U937 cells stably expressing control (CTRL) shRNA or PKR shRNA were treated with human IFNγ for the indicated times, and cell lysates were resolved by SDS-PAGE and immunoblotted with anti-phospho-Thr-202/Tyr-204-Erk or anti-Erk, as indicated. B, U937 cells stably expressing control shRNA or PKR shRNA were treated with human IFNγ for the indicated times, and cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies against phosphor-JNK or GAPDH as indicated (top two panels). Equal amounts of cell lysates from the same experiment were resolved separately by SDS-PAGE and immunoblotted with antibodies against JNK or GAPDH, as indicated (bottom two panels). C, U937 cells stably expressing CTRL shRNA or PKR shRNA were treated with TNFα for the indicated times, and cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies against phosphor-JNK or GAPDH as indicated (top two panels). Equal amounts of cell lysates from the same experiment were resolved separately by SDS-PAGE and immunoblotted with antibodies against JNK or GAPDH, as indicated (bottom two panels).