FIGURE 6.

CD spectroscopy of the LZ5 K309C/C335A mutant. A, CD spectrum at T = 278 K. The spectrum of the reduced protein is shown in red, the spectrum of the oxidized protein is shown in blue. B, thermal unfolding curves. The measured data is shown in red boxes for the reduced sample and in blue boxes for the oxidized sample. The fitted curves are shown for both samples as thin black lines. Melting temperatures obtained from a fit to a two state unfolding equation are 57.7 ± 0.3 °C for the oxidized sample and 24.3 ± 0.3 °C for the reduced sample.