TABLE 1.

Half-lives of thermal processes at 55 °C

	τ½
	WT	E181Q	S186A
	min
Thermal stability of rhodopsin
Thermal decay, A500	70 ± 2	2.3 ± 0.1	0.9 ± 0.1
Thermal isomerization, 11-cis-retinal	88 ± 10	3.8 ± 0.6	1.3 ± 0.3
Hydrolysis, A440	73 ± 3	2.6 ± 0.3	1.4 ± 0.5
Catalytic property of opsin protein
Thermal isomerization of 11-cis-retinal in the presence of opsin protein	33 ± 2	42 ± 3	29 ± 3
Thermal isomerization of free 11-cis-retinal in solution		610 ± 170