FIGURE 5.

Effect of LOX inhibition on PDGFR-β-mediated signaling. Western blot analysis of PDGF-Rβ downstream targets Akt and ERK1/2 in MKs. Bone marrow cells were stimulated with 25 ng/ml TPO for 3 days in the presence or absence of 100 μm BAPN, followed by magnetic activated cell sorting purification of MKs that were further cultured in serum-free media in the presence or absence of 100 μm BAPN for 16 h. MKs were next stimulated with 50 ng/ml PDGF-BB and collected for analysis at different time points. Blots were probed with the indicated antibodies, with t indicating total protein and p indicating the phosphorylated form. Probing with anti-β-actin was used as loading control. Shown here is a representative Western blot out of three independent experiments.