FIGURE 7.

Effect of LOX inhibition on splenic GATA-1^low MKs. Spleens were harvested from wild-type and GATA-1^low mice (male littermates) that were in either control or BAPN-drinking water treatment as described under “Experimental Procedures.” A, measurement of spleen mass. Standard deviation bars represent the mean of n = 6 mice per group (wild-type/BAPN groups, n = 4 mice); **, p < 0.001. B, representative spleen sections stained with hematoxylin and eosin. MKs were readily detected based on the large nucleus and morphology, and our method of detection was further confirmed by marking MKs with anti-CD41 as shown in supplemental Fig. S7. Original magnification, 200×. C, quantification of spleen MKs in a total area of 0.21 mm². Results are presented as the mean of three mice per group, with nine slides analyzed per mouse; *, p < 0.05.