FIGURE 6.
Gas8 morphants show slow muscle defects. Analysis of slow muscle cell nuclei (Prox1, green) and engrailed+ muscle pioneer cells (4d9, red) in 27 hpf embyos injected with Gas8 CT MO (A), Gas8 MO (B), Gas8 MO, and dnPKA mRNA co-injection (C), β-arrestin2 MO (D), Pol MO (E) as well as SmoS294 mutants (F). G and H, bargraphs represent numbers of Prox1+ cells (G) and engrailed+ cells (H) ± S.E. measured in several somites of at least 9 embryos in each condition. Gas8 MO leads to a reduction in both slow muscle cells and muscle pioneer cells (B, G, and H), similar to the effect of β-arrestin2 MO. The phenotype is milder than in SmoS294 mutant somites and it could be fully rescued by dnPKA mRNA. Embryos are shown in lateral view, anterior to the left. *, p < 0.05; **, p < 0.01, two-tailed Student's t-test compared with the non-injected wt.