Figure 3.

Antisense-correlated splicing events at the MSH6 gene. (A) The MSH6 (+ strand) and FBXO11 (− strand) known SAS locus is represented at the top of the panel, with dotted lines indicating the area of interest displayed in the bottom panel. MSH6 isoforms are depicted as thick (coding) or thin (noncoding) black bars (exons) connected by lines (introns) with directional arrows (“>” indicates + strand). The span of the longest known FBXO11 isoform is depicted on the − strand (“<”). Three MSH6 probesets have either positive (r = 0.56; blue rectangle) or negative (r = −0.59; r = −0.63; red rectangles) correlations between their respective SI values (i.e., probeset inclusion in the mRNA) and the antisense gene expression (i.e., FBXO11 expression). Probeset locations correspond to their genomic location in the MSH6 locus. In B and C the probeset SI values (left-hand y-axis) are shown along with the antisense gene expression (right-hand y-axis) for all 176 LCL samples (x-axis); samples are ordered by increasing FBXO11 expression. Trendlines (log₂) for each data series are superimposed on the graphs.