Fig. 7.

Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.