Fig. 6.

Fluorescence and interference images obtained near the ventral plasma membrane of fixed cells. (A) Immunofluorescence image showing F-actin localization near the periphery of the cell. (scale bar: 5μm). (B) Immunofluorescence image of the density of paxillin. (C) Fringe visibility and (D) height of the reflective features above the coverslip-buffer interface at discrete scan points that cover the same region as the fluorescence images calculated using the inverse method (Eqs. (8) and (9)). The height, z, was determined from the phase, θ, as: $z\hspace{0.17em}=\hspace{0.17em}f\frac{\lambda }{4\pi n_b}\theta$ with λ = 0.6328 μm, n_b = 1.333 and f = 1.2. Using this method, the height map represents the distance above the coverslip.