Table 1.
C. truncatum unigenes encoding secretory proteins
| Unique sequence ID | Accession | Putative function | Organism | E value |
|---|---|---|---|---|
| Cell envelop associated protein | ||||
| Contig 1 | XP_002144203 | GPI anchored serine-threonine rich protein | Penicillium marneffei | 9e-11 |
| Contig 2 | XP_001269791 | GPI anchored serine-threonine rich protein | Aspergillus clavatus | 1e-06 |
| Contig 3 | XP_002148880 | GPI anchored protein, putative | Penicillium marneffei | 1e-14 |
| Contig 4 | XP_002850839 | GPI anchored serine-rich protein | Microsporum canis | 5e-11 |
| Ct21-4350 | XP_002144203 | GPI anchored serine-threonine rich protein | Aspergillus fumigatus | 8e-11 |
| Ct21-949 | EEY14502 | GPI-anchored cell wall organization protein Ecm33 | Verticillium albo-atrum | 4e-49 |
| Ct21-1020 | EEY23888 | GPI transamidase component Gpi16 | Verticillium albo-atrum | 4e-100 |
| Ct21-3268 | XP_750946 | CFEM domain-containing protein | Glomerella graminicola | 4e-40 |
| Contig 7 | CAQ16271 | Hypothetical protein | Glomerella graminicola | 1e-20 |
| Ct21-4487 | CAQ16270 | Hypothetical protein | Glomerella graminicola | 7e-18 |
| Contig 9 | XP_001557501 | Predicted protein | Botryotinia fuckeliana | 9e-09 |
| Ct21-2424 | CAA04765 | Glycoprotein CIH1 | Glomerella lindemuthiana | 2e-27 |
| Ct21-2435 | CAA04765 | Glycoprotein CIH1 | Glomerella lindemuthiana | 2e-22 |
| Ct21-3485 | CAA04765 | Glycoprotein CIH1 | Glomerella lindemuthiana | 8e-29 |
| Ct21-1783 | XP_960686 | Clock-controlled protein 6 | Neurospora crassa | 1e-12 |
| Ct21-4525 | XP_960686 | Clock-controlled protein 6 | Neurospora crassa | 1e-11 |
| Contig 29 | XP_960686 | Clock-controlled protein 6 | Neurospora crassa | 3e-11 |
| Ct21-3233 | XP_002340703 | Extracellular conserved serine-rich protein | Talaromyces stipitatus | 1e-19 |
| Contig 11 | XP_746687 | Conserved glycine-rich protein | Aspergillus fumigatus | 3e-22 |
| Ct21-160 | XP_775248 | Hypothetical protein | Glomerella graminicola | 9e-08 |
| Ct21-3817 | XP_365520 | Hypothetical protein | Magnaporthe grisea | 1e-50 |
| Ct21-3527 | XP_001910524 | Hypothetical protein | Podospora anserina | 2e-05 |
| Contig 28 | XP_001264578 | MFS sugar transporter | Neosartorya fischeri | 6e-87 |
| Contig 30 | XP_389647 | Glucose-regulated protein homolog precursor GRP 78 | Gibberella zeae | 0.0* |
| Ct21-2300 | AAM00019 | ToxB precursor | Pyrenophora tritici-repentis | 3e-04 |
| Ct21-3042 | EEY18399 | Cu-Zn superoxide dismutase | Verticillium albo-atrum | 1e-45 |
| Ct21-3890 | EEY18083 | Peptidyl-prolyl cis-trans isomerase B | Verticillium albo-atrum | 3e-81 |
| Ct21-4099 | EEY20068 | Chitin deacetylase | Verticillium albo-atrum | 8e-83 |
| Ct21-4275 | XP_001831864 | Metalloreductase | Coprinopsis cinerea | 4e-14 |
| Ct21-1979 | EEY19380 | Integral membrane protein | Verticillium albo-atrum | 3e-43 |
| Contig 12 | XP_001553407 | Predicted protein | Botryotinia fuckeliana | 0.015* |
| Ct21-43 | EEY14873 | Conserved hypothetical protein | Verticillium albo-atrum | 6e-69 |
| Ct21-2181 | XP_001906422 | Hypothetical protein | Podospora anserina | 1e-23 |
| Ct21-1105 | XP_387661 | Hypothetical protein | Gibberella zeae | 9e-39 |
| Ct21-2709 | XP_387661 | Hypothetical protein | Gibberella zeae | 1e-35 |
| Ct21-3808 | XP_364081 | Hypothetical protein | Magnaporthe grisea | 9e-21 |
| Ct21-4329 | XP_001592593 | Hypothetical protein | Sclerotinia sclerotiorum | 5e-18 |
| Candidate effectors | ||||
| Contig 6 | EEY19659 | Conserved hypothetical protein | Verticillium albo-atrum | 7e-29 |
| Contig 8 | ABE73692 | Eliciting plant response-like protein | Hypocrea atroviridis | 2e-45 |
| Contig 10 | XP_386615 | Hypothetical protein | Gibberella zeae | 2e-80 |
| Contig 31 | EEU39436 | Small secreted protein | Nectria haematococca | 1e-07 |
| Ct21-741 | EEY14856 | Conserved hypothetical protein | Verticillium albo-atrum | 9e-36 |
| Ct21-1631 | EEY16152 | Conserved hypothetical protein | Verticillium albo-atrum | 5e-59 |
| Contig 32 | ABK76310 | Hypersensitive response-inducing protein | Ceratocystis ulmi | 7e-23 |
| Ct21-2867 | EEU38174 | Hypothetical protein | Nectria haematococca | 4e-36 |
| Ct21-4630 | EEU46355 | Predicted protein | Nectria haematococca | 1e-45 |
| Contig 5 | ACF19427 | Extracellular protein 6 | Passalora fulva | 0.028* |
| Ct21-1573 | ACF19427 | Extracellular protein 6 | Passalora fulva | 1e-22 |
| Hydrolases | ||||
| Contig 13 | EEY15172 | Aminopeptidase Y precursor, putative | Verticillium albo-atrum | 5e-75 |
| Contig 14 | XP_956796 | Carboxypeptidase Y precursor | Neurospora crassa | 9e-108 |
| Contig 15 | ACM42424 | Subtilisin serine protease | Chaetomium globosum | 1e-61 |
| Ct21-125 | B8XGR2 | Carboxypeptidase 2 | Trichophyton equinum | 4e-19 |
| Ct21-572 | EEY23108 | Serin endopeptidase | Verticillium albo-atrum | 1e-42 |
| Ct21-708 | AAS45251 | Subtilisin-like serine protease | Verticillium dahliae | 4e-27 |
| Ct21-1114 | XP_002843648 | Glutamate carboxypeptidase 2 | Arthroderma otae | 5e-110 |
| Ct21-1766 | EEY22919 | Peptidase M14 | Verticillium albo-atrum | 4e-99 |
| Ct21-1999 | XP_001940792 | Carboxypeptidase Y precursor | Pyrenophora tritici-repentis | 5e-44 |
| Ct21-2223 | EEY15034 | Metalloprotease | Verticillium albo-atrum | 1e-49 |
| Ct21-2830 | ACV96842 | Aspartic proteinase | Botryotinia fuckeliana | 4e-63 |
| Ct21-3428 | CAL25580 | Serin endopeptidase | Hypocrea lixii | 2e-72 |
| Ct21-3969 | CAB44651 | Chymotrypsin | Metarhizium anisopliae | 3e-62 |
| Ct21-4220 | EEY23006 | Conserved hypothetical protein | Verticillium albo-atrum | 2e-88 |
| Ct21-4840 | EEY23418 | Serin endopeptidase | Verticillium albo-atrum | 2e-22 |
| Ct21-4964 | EEY21352 | Aminopeptidase Y | Verticillium albo-atrum | 1e-52 |
| Contig 16 | EDP48344 | Pectin lyase B | Aspergillus fumigatus | 5e-75 |
| Contig 17 | XP_001935104 | Cutinase precursor | Pyrenophora tritici-repentis | 6e-60 |
| Contig 18 | O94218 | Xyloglucan-specific endo-beta-1,4-glucanase precursor | Aspergillus aculeatus | 5e-37 |
| Contig 19 | ABC65824 | Cell wall glucanosyltransferase | Metarhizium anisopliae | 3e-65 |
| Contig 20 | EEY20211 | Cutinase-2 | Verticillium albo-atrum | 3e-38 |
| Contig 21 | XP_002382421 | Esterase, putative | Aspergillus flavus | 2e-26 |
| Ct21-1152 | XP_002373471 | Lipase/esterase, putative | Aspergillus flavus | 9e-25 |
| Ct21-1372 | XP_002379112 | Esterase, putative | Aspergillus flavus | 6e-46 |
| Ct21-2524 | EEY22945 | Hydrolase (Esterase/Lipase domain) | Verticillium albo-atrum | 3e-27 |
| Ct21-2039 | EEY15257 | Pectinesterase | Verticillium albo-atrum | 1e-78 |
| Contig 25 | CAC29255 | Pectin methyl esterase | Botryotinia fuckeliana | 1e-14 |
| Contig 22 | EEY23761 | Pectate lyase | Pyrenophora tritici-repentis | 6e-79 |
| Contig 23 | XP_001933274 | Endoglucanase II | Pyrenophora tritici-repentis | 5e-61 |
| Contig 24 | XP_001940096 | Glycosyl hydrolase | Pyrenophora tritici-repentis | 5e-42 |
| Contig 26 | EEU40344 | Glycoside hydrolase family 43 | Nectria haematococca | 2e-117 |
| Contig 27 | XP_001261592 | Pectate lyase, putative | Neosartorya fischeri | 3e-39 |
| Ct21-435 | EEY22330 | Cell wall glycosyl hydrolase | Verticillium albo-atrum | 2e-137 |
| Ct21-551 | XP_001940634 | Periplasmic beta-glucosidase precursor | Pyrenophora tritici-repentis | 2e-98 |
| Ct21-605 | EEY19750 | Septation protein SUN4 | Verticillium albo-atrum | 3e-42 |
| Ct21-657 | EEU42734 | Glycoside hydrolase family 76 | Nectria haematococca | 7e-46 |
| Ct21-658 | XP_001939866 | Arabinosidase | Pyrenophora tritici-repentis | 3e-177 |
| Ct21-817 | EEY20388 | Cell wall glycosyl hydrolase | Verticillium albo-atrum | 2e-120 |
| Ct21-1102 | NP_592836 | Cell wall protein Asl1, O-glucosyl hydrolase | Schizosaccharomyces pombe | 1e-12 |
| Ct21-1170 | CAC14022 | Endopolygalacturonase | Colletotrichum gloeosporioides f. sp. malvae | 1e-78 |
| Ct21-1507 | ABC65824 | Cell wall glucanosyltransferase | Metarhizium anisopliae | 8e-56 |
| Ct21-1571 | EEY17994 | Beta-glucanase | Verticillium albo-atrum | 3e-109 |
| Ct21-1584 | AAQ23181 | Extracellular lipase | Gibberella zeae | 5e-66 |
| Ct21-1644 | EEY23761 | Pectate lyase | Verticillium albo-atrum | 3e-50 |
| Ct21-1737 | EEY19876 | Beta-glucosidase | Verticillium albo-atrum | 1e-3 |
| Ct21-1738 | EEY23761 | Pectate lyase | Verticillium albo-atrum | 3e-50 |
| Ct21-1739 | XP_001937817 | Fungal cellulose binding domain containing protein | Pyrenophora tritici-repentis | 3e-34 |
| Ct21-3571 | XP_001937817 | Fungal cellulose binding domain containing protein | Pyrenophora tritici-repentis | 9e-35 |
| Ct21-4548 | XP_001937817 | Fungal cellulose binding domain containing protein | Pyrenophora tritici-repentis | 3e-40 |
| Ct21-1786 | EEY20352 | Phosphoserine phosphatase | Verticillium albo-atrum | 2e-112 |
| Ct21-1816 | BAH10648 | Beta-L-arabinopyranosidase/alfa-D-galactopyranosidase | Fusarium oxysporum | 2e-81 |
| Ct21-1965 | EEU44929 | Glycoside hydrolase family 12 | Nectria haematococca | 4e-40 |
| Ct21-1989 | ACZ06599 | Exopolygalacturonase | Fusarium oxysporum f. sp. lycopersici | 1e-96 |
| Ct21-2762 | XP_001216041 | Endoglucanase I precursor | Aspergillus terreus | 7e-09 |
| Ct21-2876 | XP_958254 | Endoglucanase IV precursor | Neurospora crassa | 5e-56 |
| Ct21-3095 | EEY21635 | Mannan endo-1,4-beta-mannosidase | Verticillium albo-atrum | 2e-103 |
| Ct21-3342 | EEY20111 | Glycosyl hydrolase family 43 protein | Verticillium albo-atrum | 5e-119 |
| Ct21-3508 | AAM77705 | Endoglucanase | Nectria ipomoeae | 6e-66 |
| Ct21-3569 | ABH03046 | Pectin lyase I | Penicillium occitanis | 4e-63 |
| Ct21-3767 | XP_001259883 | Extracellular cellulase CelA, putative | Neosartorya fischeri | 3e-54 |
| Ct21-3948 | EEY21541 | Alpha-galactosidase | Verticillium albo-atrum | 1e-115 |
| Ct21-4357 | XP_001937210 | Beta-1,6-galactanase | Pyrenophora tritici-repentis | 1e-17 |
| Ct21-4443 | XP_001836535 | Endoglucanase B | Coprinopsis cinerea | 6e-43 |
| Other proteins | ||||
| Ct21-153 | AAL08969 | Glycine-rich protein | Coccidioides posadasii | 1e-27 |
| Ct21-1373 | EFQ36857 | NUDIX-domain containing protein | Glomerella graminicola | 1e-46 |
| Ct21-1408 | CAD71220 | Chloroperoxidase related protein | Neurospora crassa | 1e-46 |
| Ct21-3930 | EEH44533 | Glutaminase | Paracoccidioides brasiliensis | 6e-62 |
| Ct21-4401 | EEY18112 | L-ascorbate peroxidase | Verticillium albo-atrum | 4e-06 |
| Ct21-4758 | EEY17786 | Predicted protein (NAD(P)(+)-binding protein region) | Verticillium albo-atrum | 0.006* |
| Ct21-3336 | XP_360331 | Predicted protein | Magnaporthe grisea | 6e-05 |
| Ct21-25 | EEY16153 | Conserved hypothetical protein | Verticillium albo-atrum | 3e-60 |
| Ct21-695 | EEU39144 | Hypothetical protein | Nectria haematococca | 2e-21 |
| Ct21-1015 | EEU42792 | Hypothetical protein | Nectria haematococca | 4e-23 |
| Ct21-1283 | CAQ16193 | Hypothetical protein | Glomerella graminicola | 2e-05 |
* Unigenes with E value > 10-5 are classified according to their conserved signatures as described in the text.