Figure 4. Western blot analysis of the large subunits of NAR and FDH-N.

Crude extracts (25 µg protein) of MC4100, FM460 (ΔselC), PB1000 and BL21(DE3) bearing plasmids p13fnr (fnr ⁺), pCH21 (fnr ⁺), p7modE (modE ⁺) or supplemented with 500 µM nickel(II)-chloride (Ni), 15 mM formate (F) or 1 mM molybdate (MoO), when indicated were separated on 10% (w/v) SDS-PAGE after anaerobic growth in TGYEP, pH 6.5 with 100 mM potassium-nitrate. and treated with antiserum raised against A: Nar or B: FdnG.