Table 2. Specific activities of Nitrate reductase and Formate dehydrogenase N (FDH-N).
| Strain/Condition1 | Specific Nitrate reductase activity in U mg protein−1 ± standard deviation | Specific FDH-N Activity in U mg protein−1 ± standard deviation |
| MC4100 | 0.60±0.28 | 0.39±0.12 |
| BL21(DE3) | 0.01±0.02 (<0.01) | <0.01 (<0.01) |
| BL21(DE3)/pCH21 (fnr +) | <0.01 (0.54±0.17) | <0.01 (<0.01) |
| BL21(DE3)/p1fnr | 0.02±0.02 (0.40±0.23) | <0.01 (<0.01) |
| BL21(DE3)/p10fnr | <0.01 (0.08±0.08) | <0.01 (0.01±0.01) |
| BL21(DE3)/p13fnr | <0.01 (0.11±0.08) | <0.01 (<0.01) |
| BL21(DE3)/p7modE | <0.01 (<0.01) | <0.01 (0.05±0.04) |
| BL21(DE3)/p7modE/p13fnr | <0.01 (<0.01) | <0.01 (0.04±0.03) |
| BL21(DE3)/pJW3563 (selB +)/p1fnr | 0.02±0.01 (0.11±0.24) | <0.01 (0.01±0.002) |
| BL21(DE3)/pJW1753 (selD +)/p1fnr | 0.03±0.01 (0.13±0.12) | <0.01 (<0.01) |
| PB1000 | 0.01±0.01 | 0.04±0.02 |
| PB1000/pCH21 | 0.59±0.15 | 0.19±0.03 |
| PB1000/p1fnr | 0.47±0.15 | 0.24±0.05 |
| PB1000/p10fnr | 0.02±0.02 | 0.16±0.09 |
| PB1000/p13fnr | 0.16±0.06 (0.13±0.01) | 0.15±0.08 (0.16±0.06) |
1
Cells were grown in TGYEP pH 6.5 supplemented with 100 mM KNO3. Values in parenthesis were obtained when cells were grown in the presence of 1 mM sodium molybdate.