Figure 6. The apoptosis of NPCs under normoxic and hypoxia-reoxygenation conditions.

For HR treatment, NPCs were exposed 1% to O₂ for 24 h and then switched to 21% O₂ for 1, 3, 7, and 14 days, while for normoxic treatment, NPCs were consistently cultured under normoxia with 21% O₂. A. Representative immunofluorescent imaging of Cleaved caspase-3 in NPCs. Green color: Cleaved caspase-3 staining; Nuclear localization of Cleaved caspase-3 was verified by co-localization with DAPI staining (blue color). B. Statistical analysis on the relative percentage of Cleaved caspase-3 positive cells in NPCs (Mean ± S.D., n = 6).