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. 2011 Aug 3;6(8):e22901. doi: 10.1371/journal.pone.0022901

Figure 11. Effects of siRNA Cav-1 specific knockdown on the expressions of Tuj-1, VEGF in NPCs with or without V1 treatment under normoxic condition.

Figure 11

A short interfering RNA transfection was used to knock down the expression of Cav-1 in NPCs. NPCs were transfected with Cav-1 Stealth™ RNAi. All data were obtained at day 14. A. Representative results of the expressions of Cav-1, Tuj-1 and VEGF. Left, the expression of Cav-1 was successfully knocked down by the Cav-1 RNAi; Right, immunofluorescent imaging of VEGF and Tuj-1, Green color: VEGF staining. Red color: Tuj-1 staining. Nuclear localization of VEGF and Tuj-1 was verified by co-localization with DAPI staining (blue color). NC, negative control group; siCav-1, Cav-1 RNA silencing group; B. Statistical analysis on the relative percentage of VEGF and Tuj-1 positive cells in NPCs (Mean ± S.D., n = 6). siCav-1 versus NC, **p<0.01.