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. 2011 Aug 3;6(8):e22901. doi: 10.1371/journal.pone.0022901

Figure 15. Effects of Cav-1 scaffolding domain peptide on the expressions of Tuj-1 and VEGF protein in NPCs under hypoxia-reoxygenation condition.

Figure 15

Similar experimental protocols as Figure 9 were used in the experiments and data were obtained from the samples at day 14. A–B: Representative immunofluorescent imaging of Tuj-1 and VEGF in the hypoxic NPCs at day 14. Red color: Tuj-1 and VEGF staining; Tuj-1 and VEGF staining (red color) were identified in blank control group (a, b, c), control peptide group (d, e, f) and Cav-1 peptide group (g, h, i). Nuclear localizations of Tuj-1 and VEGF were verified by co-localization with DAPI staining (blue color). C–D. Statistical analysis on the relative percentage of Tuj-1 and VEGF positive cells in NPCs (Mean ± S.D., n = 6). Blank, blank control group; Con P, Cav-1 scrambled control peptide; Cav P, Cav-1 scaffolding domain peptide group. Cav P versus blank, ** p<0.01; Cav P versus Con P, ## p<0.01.