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. 2011 Aug 3;6(8):e22781. doi: 10.1371/journal.pone.0022781

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Hara et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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NAMPT (A, 15 ng) and NAPRT (B, 20 ng) were incubated with 20 µM [¹⁴C]Nam and [¹⁴C]NA for 6 and 7 min, respectively, in the presence of 1 µM PRPP and indicated concentrations of ATP. The amounts of NMN (A) and NaMN (B) formed were determined. Data in figures 2 and 3 are representative of at least three experiments.