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. 2011 Aug 3;6(8):e23191. doi: 10.1371/journal.pone.0023191

Table 2. Primers for mutants' construction and multiplex PCR.

Primers ID Nucleotide sequence (5′ end to 3′ end) Predicted product size (bp) Source or reference
stx1_A GGGTCCGGACGGTCATATGT 827 This study
stx1_B gaagcagctccagcctacacTCAGTGAAAATAGCAGGCGC
stx1_C ctaaggaggatattcatatGACCCCCTGAAGGACGGCGTTTT 814 This study
stx1_D CACCCATTGCCGCCGGATTT
stx2_A CATGCTGATGATGCTGGGAGTG 781 This study
stx2_B gaagcagctccagcctacacGCGCGTTGTACTGGATTCGA
stx2_C ctaaggaggatattcatatGAACCTGATTCGTGGTATGTGGG 801 This study
stx2_D TGGATCAGGGCTGTCGAATG
eae_A GCAATAACCAAATCATATCCGC 852 This study
eae_B gaagcagctccagcctacacAACCACCCCGGCTAAAATATGT
eae_C ctaaggaggatattcatatGCTCGAGTTTTTCAGGGGTAGCA 799 This study
eae_D TCCAGCATAGGGACCGTGCA
P1 GTGTAGGCTGGAGCTGCTTC 1463 or 1014 Datsenko and Wanner, 2000
P2 CATATGAATATCCTCCTTAGTTCC
stx1_F ATAAATCGCCATTCGTTGACTAC 180 Paton and Paton, 1998
stx1_R AGAACGCCCACTGAGATCATC
stx2_F GGCACTGTACTGAAACTGCTCC 255 Paton and Paton, 1998
stx2_R TCGCCAGTTATCTGACATTCTG
eae_F GACCCGGCACAAGCATAAGC 384 Paton and Paton, 1998
eae_R CCACCTGCAGCAACAAGAGG