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. 2011 Aug 3;6(8):e23317. doi: 10.1371/journal.pone.0023317

Figure 1. Treatment with rVP1 inhibits growth and induces apoptosis in HCC cell lines.

Figure 1

(A) Cells were treated with various concentrations of rVP1 in serum-free medium for 16 h and assayed for viability. All assays were performed in triplicate, and data shown are representative of three independent experiments. (B) Flow cytometric detection of apoptosis by annexin V and propidium iodide (PI). Cells were treated with or without 1 µM rVP1 in serum-free medium for 16 h. (C) Western blots of phospho-Akt (pAkt) and cleavage of pro-caspase-9, -3 and -7. BNL and AML12 cells were treated with 1 µM rVP1 for the times indicated. (D) Dose-dependent effect of rVP1 on pAkt and cleavage of pro-caspase-3 in BNL and Hepa1-6 cells. Cells were treated with indicated concentrations of rVP1 for 1 and 4 h for pAkt and caspase 3 detection, respectively.