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. 2011 Aug 3;6(8):e23317. doi: 10.1371/journal.pone.0023317

Figure 2. Bax is activated and dissociates from Ku70 in response to rVP1 treatment.

Figure 2

(A) Akt inhibits rVP1-induced conformational change of Bax. BNL cells were transfected with either wild-type (WT) or dominant active Akt (DA) and cultured for 24 h. Cells were then treated with or without 1 µM rVP1 diluted in serum-free medium for 1 h and analyzed by western blotting using 6A7 monoclonal antibody (detecting conformationally active Bax). (B) The level of Ku70 decreases after rVP1 treatment. BNL and Hepa1-6 cells were treated with rVP1 diluted in medium containing 1% FBS, as indicated, for 24 h. (C) rVP1 treatment diminishes the interaction between Ku70 and Bax. BNL cells were treated with or without 1 µM rVP1 in serum-free medium for 1 h and Hepa1-6 cells were treated with 2 µM rVP1 diluted in medium containing 1% FBS for 4 h. Cell lysates were immunoprecipitated with anti-Bax antibody and immunoblots were probed with anti-Ku70 antibody.