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. 2011 Aug 3;6(8):e23262. doi: 10.1371/journal.pone.0023262

Figure 5. Expression of TRAPPC4 inflenced cell growth and its depletion induced apoptosis on SW1116.

Figure 5

A: CCK-8 assay. SW1116 cells were seeded in a 96-well plate until subconfluent. Viable cells were determined by CCK-8 assay. Three independent time were performed in triplicate. Cells were treated with TRAPPC4 siRNA to knockdown TRAPPC4 expression or with Negtive Control siRNA used as control (a), as well as tranfered with pCDEF-myc-TRAPPC4 vector to overexpress TRAPPC4 protein or with pCDEF-myc vector as control (b). *, p<0.05. B: Apoptosis analysis. After SW1116 cells were treated with TRAPPC4 siRNA or Negtive Control siRNA used as control for 48 h. Apoptosis was carried out using the Annexin V assay with Propidium iodide counterstaining allowing quantification by flow cytometry. Three independent time were performed in triplicate. The data were presented as means ± standard deviation (SD). There was significant difference between the two groups( p<0.01).