(A) Through IF, ALB positive cells (green, nuclei in blue) and CK-7 positive cells (green, nuclei in blue) were differentially produced by SP-NLCs, NSP-NLCs and SSP-NLCs. The percentages of ALB or CK-7 positive cells are shown in a column chart. (B) In contrast, AFP positive cells could be found after SP-HCCs, NSP-HCCs and SSP-HCCs induction. The data are summarized in a column chart. (C) By western blotting, fold differences in specific markers relative to GAPDH were analyzed in induced SP-NLCs, NSP-NLCs and SSP-NLCs. (D) Western blotting results of tumor-specific markers in induced SP-HCCs, NSP-HCCs and SSP-HCCs. Original magnification, 200× (A, B). (For a better interpretation of the colored figure, the reader is referred to the web version of the article).