NOS Overexpression Primarily Inhibits the JNK1–Bcl-2 Pathway to Impair Autophagy
(A–F) Immunoblot analyses with anti-nNOS, anti-iNOS, anti-eNOS, anti-phospho-JNK1, anti-phospho-Bcl-2, anti-phospho-S6K, and anti-phospho-S6 antibodies show that stable HEK293 cell lines overexpressing nNOS (A and D), iNOS-GFP (B and E), or eNOS (C and F) inhibited both the JNK1–Bcl-2 and mTORC1 pathways compared to the HEK293 control cells under basal (FM) (A–C) or starvation (HBSS) (D–F) conditions. Graphical data denote mean ± SEM.