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Reducing SDS PAGE analysis of a stressed mAb (2 weeks at 45°C, pH 9) and its SEC-purified monomer. Compared to the whole sample, the monomeric fraction lacks bands corresponding to the hinge cleavage (Fc HC and Fab HC) but contains similar bands corresponding to several cleavages within the immunoglobulin domains. Cleavage in the CH1 domain loop K133STSGGT yields two fragments of approximately 35 and 15 kDa.
