Figure 5.

Comparison of the cleavage pattern in three monoclonal antibodies. The three antibodies were stored in PBS at pH 7.0 for three months at 45°C. All antibodies show comparable cleavage in the hinge (Fc HC band, the Fab HC band is not apparent in Ab3 due to co-migration with the light chain), comparable cleavage in the K₁₃₃STSGGT loop (in agreement with the sequence of the variable domain the N-terminal fragment (CH1 band) showed different molecular weight), comparable cleavage in the CL domain. Ab3 has two additional bands that correspond to two cleavages (seen as a doublet on the small N-terminal fragment) in heavy chain CDR3.