Table 1.
Summary of residues involved in peptide bond cleavage, conditions for testing and references that discuss mechanisms of cleavage
| Residue | Peptide bond | Primary sequence | System | pH | Temperature | Reference |
| Asp | Asp-Xaa | Xaa: Gly | hexapeptide | 0.3–10 | 37°C | 14 |
| Xaa: Ser, Gly, Val | hexapeptide | 1.0, 10.0 | 70°C | 19 | ||
| Xaa: Tyr, Ser, Phe | glucagon | 1.0–2.4 | 60°C | 15 | ||
| Xaa: Pro | 18 | |||||
| Xaa:Gln, Pro, Lys, Leu | decapeptide | 4.0–5.5 | 25°C, 40°C, 60°C | 17 | ||
| Xaa: Asp | IgG2 | 5.2 | 4°C, 25°C, 45°C | 7 | ||
| Xaa-Asp | Xaa: Pro, Gly | hexapeptide | 1.0, 10.0 | 70°C | 19 | |
| Gly | Gly-Xaa | Xaa:Gly, Ala, Leu, Val | dipeptide | 12.6 | 30°C | 21 |
| Xaa-Gly | Xaa:Ala, Leu, Ser | |||||
| Thr | Gly-Thr | |||||
| Ser | Xaa-Ser | Xaa: Gly, Ala, Leu | ||||
| Xaa-Ser | alkaline | 25 | ||||
| Cys-Cys | Xaa-Cys | IgG1 | 7–10 | 45°C | 6 | |
| Asn | Asn-Xaa | Xaa: Ser, Val, Leu, Pro | hexapeptides | 7.5, 10 | 37°C | 27 |