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. 2011 May 1;3(3):264–272. doi: 10.4161/mabs.3.3.15616

Figure 4.

Figure 4

Biodistribution studies in tumor bearing mice of scFv(F8) and scFv(A9) in diabody format (A) and CRAbs (B). Both parts report the schematic representation of antibody structure, the size exclusion analysis of antibody fragments on Superdex75 (Amersham, GE) (all antibodies have a main elution peak around 10 mL, which corresponds to the expected size of 52–54 kDa) and the biodistribution analysis displayed as percent injected dose per gram (%ID/g) in tumor (Tu) and in measured mice organs: liver (Li), lung (Lu), spleen (Sp), heart (He), kidney (Ki), intestine (Int), blood (Blo). Radioactivity in tumor and organs have been counted at 24 (dark grey) and 48 (light grey) hours post intravenous injection of radiolabeled antibodies. In (B), in the SEC profile of F8-10aa linker-2H7, the peak corresponding to the monomeric CRAb is marked. Small grey arrows indicate the retention volumes of the standard proteins (from left to right: blue dextran, bovine serum albumin, ovalbumin, scFv(F8), ribonuclease A).